The bacterial bags should be turned 4 to 5 times during the indoor cultivation period. The first turn should occur 6 to 7 days after inoculation, and then every 7 to 10 days thereafter. It's important to inspect each bag carefully at regular intervals and address any issues promptly. If mild contamination is detected, you may notice small, scattered spots or filamentous microorganisms in the folds or creases of the bag. In such cases, prepare a disinfectant solution by mixing 50 ml of 75% alcohol with 30 ml of 36% formaldehyde. Inject this mixture into the affected area and gently massage the surface with your fingers to ensure the solution thoroughly covers the contaminated region. Then, seal the injection site with adhesive tape to prevent further spread.
If the contamination has entered the inoculation hole and the mycelium is still in an early growth stage, the bacterial bag can still be salvaged. First, apply a 5% to 10% lime water supernatant to the affected area, followed by a 50% more diluted bacterial solution. Be sure to avoid direct contact with shiitake mushrooms if they are being cultivated. However, if dead or heavily contaminated areas are found, the bag must be re-inoculated under sterile conditions.
In cases of severe contamination where the bag is completely covered with foreign bacteria, it's best to remove the bag, break it open, and mix the contents with a 3% lime solution. Let it sit overnight, then spread and dry the material before re-dosing, re-bagging, and sterilizing it before re-inoculation. This process helps eliminate contaminants and ensures a clean environment for the next batch. Always maintain strict hygiene practices to prevent future contamination and ensure successful mushroom cultivation.
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