Recently, the Chinese Academy of Sciences’ Overseas Outstanding Youth Fund Project, led by fellow German scholar and part-time researcher Zhang Youming of the Institute of Subtropical Agriculture, Chinese Academy of Sciences, has made important progress in the “Generation of Functional Feeds and Foods Containing Lactoferricin B in Genetic Engineeringâ€. Through research, E. Coli genetically engineered bacteria that can efficiently express bovine lactoferricin (abbreviated as Lfcin B) were successfully obtained. At present, the abuse of antibiotics to animals has become a threat to human health and the continued healthy production of animals. Therefore, scientists must find a safe alternative to new antibiotics to solve this problem. Bovine lactoferricin is composed of 25 amino acid residues and is produced by the degradation of lactoferrin in bovine milk by proteases. It has a variety of physiologically active functions, and is not easy to produce drug resistance. It has broad application prospects of novel antimicrobial peptides. However, the natural separation of Lfcin B is low in yield, time-consuming, complicated and expensive. The cost of artificially synthesized Lfcin B and its derivatives is higher and it cannot be produced on a large scale, which restricts the application of Lfcin B. The use of genetic engineering techniques to transfer Lfcin B gene into other organisms is the most promising way to solve the problem of Lfcin B deficiency in industrial use. However, the domestic research on the Lfcin B gene hierarchy started after 2002, and the Lfcin B bioreactor None of the builds were successful. Therefore, it is of great significance to construct genetically engineered bacteria and produce Lfcin B. Last year, during his working visit to the Institute of Subtropical Agriculture Ecology, Dr. Zhang Youming established the research direction with Yin Yulong, a researcher at the Institute of Livestock and Poultry Health and Aquaculture Research. After the project has not yet been formally approved, the company will raise funds for its own research. The team led by Zhang Youming used the Red/ET recombination technology invented during his work in the European Molecular Biology Laboratory to re-encode the fully synthetic Lfcin B gene fragment into a plasmid, which was then transformed into BL21 E. coli for expression. . In the process of gene recombination, Zhang Youming designed the anti-chloramphenicol gene linked to the Lfcin B gene, greatly improving the screening efficiency of the target gene into positive bacteria. Due to the strong bactericidal effect of Lfcin B, the Lfcin B gene transferred into E.coli, once expressed, will form a suicide effect and cannot continue to be produced. The research team adds the suppressor T7 promoter gene in the genome of the transgenic engineering strain to reproduce normally. When it grows, it does not express Lfcin B gene, but when it needs to be expressed, a little inducer is added, that is, the T7 promoter is immediately activated, and Lfcin B gene is expressed abundantly. The DNA sequence detection of the transgenic fragment and the bacteriostatic test of the expression product confirm Lfcin B Genes are correctly transferred and expressed in engineered bacteria. The results of this work may significantly reduce the production cost of bovine lactoferrin, making it possible to use it as an additive in the feed industry and aquaculture, and to provide the possibility for China to create new drugs with its own intellectual property rights.
Herbal Medicine,Herbal Medicine San Diego,Herbal Medicine Degree,Herbal Medicine Books
Fruit-Vegetable Powder, Herbal Medicine Co., Ltd. , http://www.nbplantextract.com