In the long-term analysis work, liquid chromatograph operators often feel that the sensitivity of the HPLC is not enough, and even suspect that the chromatograph components have problems. As a manufacturer of liquid chromatographs, we should generally consider the following aspects.
1 is not high-performance liquid chromatograph sensitivity is not enough, but the sample concentration is not enough; the solution is to increase the sample size.
2 The sample does not enter the detector, because the sample may not flow out of the column or only partially flow out, so it is often mistaken for the sensitivity of HPLC is not enough; solution: the mobile phase composition or pH can be changed according to the chemical nature of the sample ; Or swap the column.
3 high performance liquid chromatography condition setting is not appropriate, the wavelength detector set unreasonable values; Solution: The absorption peaks in the sample on the spectrometer scanned, adjust the test wavelength detector or substitute non-selective detector.
4 detector attenuation is too much; solution: adjust the detector's range to reduce attenuation.
5 detector time constant is too small; solution: increase the time parameter.
6 detector flow cell window contamination; solution: clean the flow cell window with a mobile phase of greater polarity.
7 There are bubbles in the flow cell of the detector; Solution: Flush the flow cell with a large flow of mobile phase.
8 Recorder sensitivity range is not set properly; Solution: Adjust the recorder's range setting.
9 The mobile phase flow rate is too fast; solution: adjust the mobile phase flow rate to a suitable level.
Do you have any new inspiration and thoughts on the use of liquid chromatographs through the above analysis of the sensitivity of high performance liquid chromatography? If you have any doubts or difficulties, please call the company's chromatographer. You can also go to the company's website to consult the staff online. We will answer you one by one.
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